NGM Agar 3 (+IPTG+FUDR+Carb.)

• Begin with approx. 700 mLs of DI H₂O.
• Weigh out and add ingredients with constant stirring:

• NaCl 3g
• Bacto Peptone 2.5g
• Bacto Agar 17g

• Bring up to 975 mL with DI H₂O
• Autoclave
• Allow to cool in 50˚C water bath
• Add:

• 1 M CaCl₂ 1mL (final conc. 1mM)
• 1 M MgSO₄ 1mL (final conc. 1mM)
• 1 M KPO₄ 25mL (final conc. 25mM)
• 5mg/mL cholesterol

in 95-100% ethanol 1mL

o 1 M IPTG 1mL (final conc. 1mM)

o 0.1 M FUDR 0.5mL (final conc. 0.05mM)

o 100mg/mL Carb. 1mL

Ian A. Hope (editor). C. elegans: A Practical Approach. Oxford University Press, New York, New York, 1999.