NGM Buffer

• Begin with approx. 800 mLs of DI H₂O.
• Weigh out and add ingredients with constant stirring:

• NaCl 3g
• 1 M KPO₄ 25mL (final conc. 25mM)

• Bring up to 1 L with DI H₂O
• Autoclave
• Allow to cool in 50˚C water bath
• Add:

• 1 M CaCl₂ 1mL (final conc. 1mM)
• 1 M MgSO₄ 1mL (final conc. 1mM)

 
Ian A. Hope (editor). C. elegans: A Practical Approach. Oxford University Press, New York, New York, 1999.