YNB 4

YNB (1x)

• Begin with approx. 800 mls of DI H₂O
• Weigh out and add ingredients with constant stirring:

o Yeast Nitrogen Base (without amino acids) 6.7g

• Bring up to 900 mLs with DI H₂O
• Weigh out 20g of Glucose/Dextrose and add to 100 mLs of DI H₂O
• Autoclave YNB and glucose separately
• Allow to cool in 50˚ water bath
• Combine YNB with glucose solution