YP 2

YP (1.1x) (900 mL) Agar

• Begin with approx. 700 mls of DI H₂O
• Weigh out and add ingredients with constant stirring:

• Bacto Yeast Extract 10g
• Bacto Peptone 20g

• Weigh out and add with stirring:

• Bacto Agar 20g

• Bring up to 900 mL with DI H₂O
• Separate YP into two 1L bottles of 450 mL
• Autoclave
• Allow to cool in 50˚ water bath

Verified 6/1/2005 by MaryAnn Hartman; Methods in Yeast Genomics, Cold Spring Harbor, 2000 ed.