YPD 1

YPD (1x) (1L) Agar

• Begin with approx. 700 mls of DI H₂O
• Weigh out and add ingredients with constant stirring:

• Bacto Yeast Extract 10g
• Bacto Peptone 20g

• Check pH. Adjust to 6.0 with HCl or NaOH
• Weigh out and add with stirring:

• Bacto Agar 20g

• Bring up to 900 mLs with DI H₂O
• Separate YP into two 1L bottles of 450 mLs each
• Weigh out 20g of Glucose/Dextrose and add to 100 mLs of DI H₂O
• Autoclave YP and glucose separately
• Allow to cool in 50˚ water bath
• Combine each 450mL YP with 50 mLs glucose solution

Verified 6/1/2005 by MaryAnn Hartman; Methods in Yeast Genomics, Cold Spring Harbor, 2000 ed.