YPD 4

YPD (1x) (1L)

• Begin with approx. 700 mls of DI H₂O
• Weigh out and add ingredients with constant stirring:

• Bacto Yeast Extract 10g
• Bacto Peptone 20g

• Bring up to 900 mLs with DI H₂O
• Weigh out 20g of Glucose/Dextrose and add to 100 mLs of DI H₂O
• Autoclave separately
• Allow to cool in 50˚ water bath
• Combine YP with glucose solution

Verified 6/1/2005 by MaryAnn Hartman; Methods in Yeast Genomics, Cold Spring Harbor, 2000 ed