In the spirit of organization (of which I am more a fan than a practitioner) I would like to move all of my yeast protocols from loose leaf sheet to this website. This is a work in progress; more links are coming soon.


Assays

Fluctuation Assay
A modification of the original Luria-Delbruck fluctuation assay using small-volume cultures in 96-well plates

Fitness Assay [PDF]
Assay for measuring relative growth rate using a fluorescently-labeled reference strain

Competitive Mating Assay [PDF]
Analogous to the Fitness Assay, this assay measures the relative mating ability. Although described for drug-marked strains, this protocol would be best with fluorescently-labeled strains

Genomics Protocols

Harvesting cells for RNA [PDF]

RNA prep [PDF]

RNA labeling [PDF]

Microbiology Protocols

Yeast Transformation [PDF]

PCR [PDF]

Yeast Genomic Prep [PDF]

Yeast Sporulation and Tetrad Dissection [PDF]

Coulter Counter [PDF]